摘要：目的研究miR-29b-3p靶向GRM4對前額皮質(zhì)神經(jīng)元凋亡的影響及作用機制。方法培養(yǎng)原代前額皮層神經(jīng)元,轉(zhuǎn)染miR-29b-3p的mimics和inhibitor至原代前額皮層神經(jīng)元,采用qRT-PCR及WB方法檢測miR-29b-3p及GRM4表達水平,MTT法檢測神經(jīng)元細(xì)胞活力,流式細(xì)胞技術(shù)檢測神經(jīng)元細(xì)胞凋亡,免疫雙標(biāo)法檢測GRM4與凋亡相關(guān)蛋白(cleaved caspase-3)關(guān)系;采用雙熒光素酶法檢測miR-29b-3p靶向GRM4基因的機制。結(jié)果激活miR-29b-3p表達使前額皮層神經(jīng)元細(xì)胞GRM4表達降低,神經(jīng)元細(xì)胞活力增加,細(xì)胞凋亡減少;抑制miR-29b-3p表達使前額皮層神經(jīng)元細(xì)胞GRM4表達增加,神經(jīng)元細(xì)胞活力降低,神經(jīng)元細(xì)胞凋亡增加。免疫雙標(biāo)法結(jié)果顯示,前額皮層神經(jīng)元GRM4表達水平與凋亡相關(guān)蛋白表達水平呈正相關(guān)關(guān)系;雙熒光素酶法證實GRM4是miR-29b-3p發(fā)揮作用的靶點。結(jié)論MiR-29b-3p通過抑制GRM4表達降低前額皮層神經(jīng)元凋亡。